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Genre/Form: | Thèses et écrits académiques |
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Additional Physical Format: | Relaxation des ions de sodium en RMN in vivo et ex-vivo pour la caractérisation de l'état cellulaire / Mihaela Lupu Grenoble : Atelier national de reproduction des thèses, 2003 Microfiches. ([@Grenoble thèses]) (ABES)246737468 |
Material Type: | Thesis/dissertation |
Document Type: | Book |
All Authors / Contributors: |
Mihaela Lupu; Joël Mispelter; Université Paris-Sud (1970-2019). |
OCLC Number: | 491310273 |
Description: | 145 p. : ill. ; 30 cm. |
Responsibility: | Mihaela Lupu ; sous la dir. de Joël Mispelter. |
Abstract:
For this study, the single quantum relaxation parameters (transverse relaxation times and corresponding amplitudes) as well as triple quantum filtered relaxation results were used for determining the quadrupolar sodium ions relaxation characteristics in biological systems. The retrieval of single quantum relaxation parameters was done via an original fitting method of the relaxation decays: Singular Value Decompositions. This fitting method is reliable, stable and offers a new relaxation parameter, singular values, for characterizing the state of the investigated systems. The sodium ions quadrupolar characteristics offer the possibility to distinguish via relaxation parameters the bound pools from the free compartments, co-existing in the sample, without adding any relaxation or shifting reagents. The experimental results put into evidence an important increase of extracellular binding sites, at the membrane level for perfused infected HeLa cells as compared with control. The cells apoptotic state was evidenced via NMR relaxation parameters. The same method was used for investigating the tissue pathological condition. For this purpose, normal, perfused liver was measured as compared with the same tissue while the ischemic state was induced. Irreversible tissue damage was accompanied with an important increase of bound sodium population, evidenced by both single quantum relaxation parameters as well as by TQF spectral amplitudes. The study also revealed the existence of an exchange process between the bound and free compartments modulated by the populations involved in. The overall results indicated that the increase of sodium bound populations in pathological cells or tissues may be used for clinical diagnose or treatment follow-up in a perfect non-invasive way.
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