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Review Article - Laboratory Methods for Evaluating the Effect of Low Level Laser Therapy (LLLT) In Wound Healing

Author: Hawkins, D.; Abrahamse, H.
Publisher: Ibadan Biomedical Communications Group
Edition/Format:   Downloadable archival material : English
Summary:
The basic tenet of laser therapy is that laser radiation has a wavelength dependent capability to alter cellular behaviour in the absence of significant heating. Low intensity radiation can inhibit as well as stimulate cellular activity. Laser therapy typically involves the delivery of 1-4J/cm2 to treatments sites with lasers having output powers between 10mW and 90mW. There are two major areas of laser therapy  Read more...
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Details

Genre/Form: journal
Material Type: Internet resource
Document Type: Internet Resource, Archival Material
All Authors / Contributors: Hawkins, D.; Abrahamse, H.
OCLC Number: 727690732
Language Note: English en_US
Notes: 143803 bytes
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Abstract:

The basic tenet of laser therapy is that laser radiation has a wavelength dependent capability to alter cellular behaviour in the absence of significant heating. Low intensity radiation can inhibit as well as stimulate cellular activity. Laser therapy typically involves the delivery of 1-4J/cm2 to treatments sites with lasers having output powers between 10mW and 90mW. There are two major areas of laser therapy research: the laboratory and the clinic. The laboratory presents the least ambiguous results. Here, although unsupported results do appear, the vast majority of published work finds clear evidence that laser irradiation alters cellular processes in a nonthermal, wavelength-dependent manner. Low energy laser irradiation alters the cellular function by effecting protein synthesis, cell growth and differentiation, cell motility, membrane potential and binding affinities, neurotransmitter release, ATP synthesis and prostaglandin synthesis. Laboratory findings provide scientific rationale of laser therapy and the effect of laser therapy on cellular processes. This review outlines some of the current methods employed in the laboratory to measure the effect of low level laser therapy (LLLT) on cellular and molecular processes in the cell. This review briefly explains the different structural, cellular and molecular parameters and highlights some of the basic principles and protocols including specialized equipment requirements.

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