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Schwann cells : methods and protocols

Author: Paula V Monje; Haesun A Kim
Publisher: New York, NY : Humana Press : Springer, [2018] ©2018
Series: Methods in molecular biology (Clifton, N.J.), v. 1739.
Edition/Format:   eBook : Document : EnglishView all editions and formats

This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. It also contains protocols to create cancer cell lines and engineered Schwann  Read more...


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Genre/Form: Laboratory Manual
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Schwann cells.
New York, NY : Humana Press : Springer, [2018]
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Paula V Monje; Haesun A Kim
ISBN: 9781493976492 1493976494
OCLC Number: 1028749157
Description: 1 online resource (xiii, 488 pages) : illustrations (some color)
Contents: Isolation of schwann cell precursors from rodents / Rhona Mirsky and Kristjan R. Jessen --
Preparation of neonatal rat schwann cells and embryonic dorsal root ganglia neurons for in vitro myelination studies / Patrice Maurel --
Isolation and expansion of schwann cells from transgenic mouse models / Jihyun Kim and Haesun A. Kim --
Isolation, culture, and cryopreservation of adult rodent schwann cells derived from immediately dissociated teased fibers / Natalia D. Andersen and Paula V. Monje --
Detailed protocols for the isolation, culture, enrichment and immunostaining of primary human schwann cells / Tamara Weiss, Sabine Taschner-Mandl, Peter F. Ambros, and Inge M. Ambros --
Magnetic-activated cell sorting for the fast and efficient separation of human and rodent schwann cells from mixed cell populations / Kristine M. Ravelo, Natalia D. Andersen, and Paula V. Monje --
Culture and expansion of rodent and porcine schwann cells for preclinical animal studies / Adriana E. Brooks, Gagani Athauda, Mary Bartlett Bunge, and Aisha Khan --
Chemical conversion of human fibroblasts into functional schwann cells / Eva C. Thoma --
Derivation of fate-committed schwann cells from bone marrow stromal cells of adult rats / Y.P. Tsui, Graham K. Shea, Y.S. Chan, and Daisy K.Y. Shum --
Human induced pluripotent stem cell-derived sensory neurons for fate commitment of bone marrow stromal cell-derived schwann cells / Sa Cai, Daisy K.Y. Shum, and Ying-Shing Chan --
Generation and use of merlin-deficient human schwann cells for a high-throughput chemical genomics screening assay / Alejandra M. Petrilli and Cristina Fernández-Valle --
Lentiviral transduction of rat schwann cells and dorsal root ganglia neurons for in vitro myelination studies / Corey Heffernan and Patrice Maurel --
Preservation, sectioning, and staining of schwann cell cultures for transmission electron microscopy analysis / Vania W. Almeida, Margaret L. Bates, and Mary Bartlett Bunge --
Scalable differentiation and dedifferentiation assays using neuron-free schwann cell cultures / Paula V. Monje --
Pseudopod system for axon-glia interactions : stimulation and isolation of schwann cell protrusions that form in response to axonal membranes / Yannick Poitelon and M. Laura Feltri --
In vitro analysis of the role of schwann cells on axonal degeneration and regeneration using sensory neurons from dorsal root ganglia / Rodrigo López-Leal, Paula Diaz, and Felipe A. Court --
Culture model to study neuron-schwann cell-astrocyte interactions / Susana R. Cerqueira, Yee-Shuan Lee, and Mary Bartlett Bunge --
Preparation of matrices of variable stiffness for the study of mechanotransduction in schwann cell development / Mateusz M. Urbanski and Carmen V. Melendez-Vasquez --
Purification of exosomes from primary schwann cells, RNA extraction, and next-generation sequencing of exosomal RNAs / Cristian De Gregorio, Paula Díaz, Rodrigo López-Leal, Patricio Manque, and Felipe A. Court --
3D cancer migration assay with schwann cells / Laura Fangmann, Steffen Teller, Pavel Stupakov, Helmut Friess, Güralp O. Ceyhan, and Ihsan Ekin Demir --
Teased fiber preparation of myelinated nerve fibers from peripheral nerves for vital dye staining and immunofluorescence analysis / Alejandra Catenaccio and Felipe A. Court --
Whole mount immunostaining on mouse sciatic nerves to visualize events of peripheral nerve regeneration / Xin-Peng Dun and David B. Parkinson --
Use of low vacuum scanning electron microscopy (LVSEM) to analyze peripheral nerve samples / Peter Bond and David B. Parkinson --
Analysis of myelinating schwann cells in human skin biopsies / Mario A. Saporta and Renata de Moraes Maciel --
Whole mount in situ hybridization and immunohistochemistry for zebrafish larvae / Rebecca L. Cunningham and Kelly R. Monk --
Transmission electron microscopy for zebrafish larvae and adult lateral line nerve / Rebecca L. Cunningham and Kelly R. Monk --
Live imaging of schwann cell development in zebrafish / Rebecca L. Cunningham and Kelly R. Monk --
Transplantation of adult rat schwann cells into the injured spinal cord / Ying Dai and Caitlin E. Hill --
Schwann cell transplantation methods using biomaterials / Christine D. Plant and Giles W. Plant --
Viral transduction of schwann cells for peripheral nerve repair / Christine D. Plant and Giles W. Plant --
Intraspinal delivery of schwann cells for spinal cord injury / Andrea J. Santamaría, Juan P. Solano, Francisco D. Benavides, and James D. Guest.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1739.
Responsibility: edited by Paula V. Monje, Haesun A. Kim.


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