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Schwann cells : methods and protocols

Author: Paula V Monje; Haesun A Kim
Publisher: New York, NY : Humana Press : Springer, [2018] ©2018
Series: Methods in molecular biology (Clifton, N.J.), v. 1739.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. It also contains protocols to create cancer cell lines and engineered Schwann  Read more...

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Genre/Form: Laboratory Manual
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Schwann cells.
New York, NY : Humana Press : Springer, [2018]
(OCoLC)1007035893
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Paula V Monje; Haesun A Kim
ISBN: 9781493976492 1493976494
OCLC Number: 1028749157
Description: 1 online resource (xiii, 488 pages) : illustrations (some color)
Contents: Isolation of schwann cell precursors from rodents / Rhona Mirsky and Kristjan R. Jessen --
Preparation of neonatal rat schwann cells and embryonic dorsal root ganglia neurons for in vitro myelination studies / Patrice Maurel --
Isolation and expansion of schwann cells from transgenic mouse models / Jihyun Kim and Haesun A. Kim --
Isolation, culture, and cryopreservation of adult rodent schwann cells derived from immediately dissociated teased fibers / Natalia D. Andersen and Paula V. Monje --
Detailed protocols for the isolation, culture, enrichment and immunostaining of primary human schwann cells / Tamara Weiss, Sabine Taschner-Mandl, Peter F. Ambros, and Inge M. Ambros --
Magnetic-activated cell sorting for the fast and efficient separation of human and rodent schwann cells from mixed cell populations / Kristine M. Ravelo, Natalia D. Andersen, and Paula V. Monje --
Culture and expansion of rodent and porcine schwann cells for preclinical animal studies / Adriana E. Brooks, Gagani Athauda, Mary Bartlett Bunge, and Aisha Khan --
Chemical conversion of human fibroblasts into functional schwann cells / Eva C. Thoma --
Derivation of fate-committed schwann cells from bone marrow stromal cells of adult rats / Y.P. Tsui, Graham K. Shea, Y.S. Chan, and Daisy K.Y. Shum --
Human induced pluripotent stem cell-derived sensory neurons for fate commitment of bone marrow stromal cell-derived schwann cells / Sa Cai, Daisy K.Y. Shum, and Ying-Shing Chan --
Generation and use of merlin-deficient human schwann cells for a high-throughput chemical genomics screening assay / Alejandra M. Petrilli and Cristina Fernández-Valle --
Lentiviral transduction of rat schwann cells and dorsal root ganglia neurons for in vitro myelination studies / Corey Heffernan and Patrice Maurel --
Preservation, sectioning, and staining of schwann cell cultures for transmission electron microscopy analysis / Vania W. Almeida, Margaret L. Bates, and Mary Bartlett Bunge --
Scalable differentiation and dedifferentiation assays using neuron-free schwann cell cultures / Paula V. Monje --
Pseudopod system for axon-glia interactions : stimulation and isolation of schwann cell protrusions that form in response to axonal membranes / Yannick Poitelon and M. Laura Feltri --
In vitro analysis of the role of schwann cells on axonal degeneration and regeneration using sensory neurons from dorsal root ganglia / Rodrigo López-Leal, Paula Diaz, and Felipe A. Court --
Culture model to study neuron-schwann cell-astrocyte interactions / Susana R. Cerqueira, Yee-Shuan Lee, and Mary Bartlett Bunge --
Preparation of matrices of variable stiffness for the study of mechanotransduction in schwann cell development / Mateusz M. Urbanski and Carmen V. Melendez-Vasquez --
Purification of exosomes from primary schwann cells, RNA extraction, and next-generation sequencing of exosomal RNAs / Cristian De Gregorio, Paula Díaz, Rodrigo López-Leal, Patricio Manque, and Felipe A. Court --
3D cancer migration assay with schwann cells / Laura Fangmann, Steffen Teller, Pavel Stupakov, Helmut Friess, Güralp O. Ceyhan, and Ihsan Ekin Demir --
Teased fiber preparation of myelinated nerve fibers from peripheral nerves for vital dye staining and immunofluorescence analysis / Alejandra Catenaccio and Felipe A. Court --
Whole mount immunostaining on mouse sciatic nerves to visualize events of peripheral nerve regeneration / Xin-Peng Dun and David B. Parkinson --
Use of low vacuum scanning electron microscopy (LVSEM) to analyze peripheral nerve samples / Peter Bond and David B. Parkinson --
Analysis of myelinating schwann cells in human skin biopsies / Mario A. Saporta and Renata de Moraes Maciel --
Whole mount in situ hybridization and immunohistochemistry for zebrafish larvae / Rebecca L. Cunningham and Kelly R. Monk --
Transmission electron microscopy for zebrafish larvae and adult lateral line nerve / Rebecca L. Cunningham and Kelly R. Monk --
Live imaging of schwann cell development in zebrafish / Rebecca L. Cunningham and Kelly R. Monk --
Transplantation of adult rat schwann cells into the injured spinal cord / Ying Dai and Caitlin E. Hill --
Schwann cell transplantation methods using biomaterials / Christine D. Plant and Giles W. Plant --
Viral transduction of schwann cells for peripheral nerve repair / Christine D. Plant and Giles W. Plant --
Intraspinal delivery of schwann cells for spinal cord injury / Andrea J. Santamaría, Juan P. Solano, Francisco D. Benavides, and James D. Guest.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1739.
Responsibility: edited by Paula V. Monje, Haesun A. Kim.

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