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Sedimentation velocity analytical ultracentrifugation : interacting systems

Author: Peter Schuck; Huaying Zhao, (Biophysicist)
Publisher: Boca Raton, FL : CRC Press, Taylor & Francis Group, [2018]
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
"Analytical ultracentrifugation is one of the most powerful solution techniques for the study of macromolecular interactions, to define the number and stoichiometry of complexes formed, and to measure affinities ranging from very strong to very weak and repulsive. Building on the data analysis tools described in the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Schuck, Peter (Peter W.).
Sedimentation velocity analytical ultracentrifugation.
Boca Raton, FL : CRC Press, Taylor & Francis Group, [2018]
(DLC) 2017016200
(OCoLC)979564762
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Peter Schuck; Huaying Zhao, (Biophysicist)
ISBN: 9781351976848 1351976842
OCLC Number: 1008958408
Description: 1 online resource (xxv, 271 pages .)
Contents: Exact description of ideally sedimenting associating systems --
Phenomenology of sedimentation of interacting systems --
Approximate solutions for the coupled Lamm equations of reacting systems --
Sedimentation coefficient distributions --
Nonideal sedimentation velocity --
Practical analysis of interacting systems.
Responsibility: Peter Schuck, Huaying Zhao.

Abstract:

"Analytical ultracentrifugation is one of the most powerful solution techniques for the study of macromolecular interactions, to define the number and stoichiometry of complexes formed, and to measure affinities ranging from very strong to very weak and repulsive. Building on the data analysis tools described in the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles, and the experimental and instrumental aspects in the first volume Basic Principles of Analytical Ultracentrifugation, the present volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems is devoted to the theory and practical data analysis of dynamically coupled sedimentation processes. Features: - The rich phenomenology of sedimenting interacting systems is systematically presented - A physical picture and mathematical framework is developed side-by-side - Strategies for practical data analysis are reviewed - Consequences for experimental design strategies are discussed - Cross-references to practical data analysis software - Provides extensive illustration of principles and examples The volume is designed to fill a gap in biophysical methodology to provide a framework that builds on the fundamentals of the highly developed traditional methods of analytical ultracentrifugation, updated with current methodology and from a viewpoint of modern applications. It is written for researchers and graduate students interested in the application of analytical ultracentrifugation for the study of interacting systems, such as biological macromolecules, multi-protein complexes, polymers, or nanoparticles"--

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"Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems should be considered essential reading for those interested in the quantitative analysis of macromolecular interactions. Read more...

 
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