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The surfaceome : methods and protocols

Author: Kenneth R Boheler; Rebekah L Gundry
Publisher: New York, NY : Humana Press : Springer, [2018] ©2018
Series: Methods in molecular biology (Clifton, N.J.), v. 1722.
Edition/Format:   eBook : Document : EnglishView all editions and formats

This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. Section 1 focuses on discovery-based approaches for cataloging surfaceome  Read more...


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Genre/Form: Laboratory Manual
Electronic books
Laboratory manuals
Additional Physical Format: Print version:
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Kenneth R Boheler; Rebekah L Gundry
ISBN: 9781493975532 1493975536 9781493975518 149397551X
OCLC Number: 1016160636
Description: 1 online resource (xi, 347 pages) : illustrations (some color)
Contents: Surfaceome analysis protocol for the identification of novel ordetella pertussis antigens / Yulanda M. Williamson, Jennifer Whitmon, Rolieria West-Deadwyler, Hercules Moura, Adrian R. Woolfitt, Jon Rees, David M. Schieltz, and John R. Barr --
"Shaving" live bacterial cells with proteases for proteomic analysis of surface proteins / Manuel J. Rodrıguez-Ortega --
Methods for mapping the extracellular and membrane proteome in the avian embryo, and identification of putative vascular targets or endothelial genes / Witold W. Kilarski, John Herbert, and Andreas Bikfalvi --
Mass spectrometry-based identification of extracellular domains of cell surface N-glycoproteins : defining the accessible surfaceome for immunophenotyping stem cells and their derivatives / Chelsea M. Fujinaka, Matthew Waas, and Rebekah L. Gundry --
Application of higher density iron oxide nanoparticle pellicles to enrich the plasma membrane and its proteome from cells in suspension / Rebecca L. Rose, Waeowalee Choksawangkarn, and Catherine Fenselau --
Proteomic profiling of secreted proteins, exosomes, and microvesicles in cell culture conditioned media / Ankit Sinha, Simona Principe, Javier Alfaro, Alex Ignatchenko, Vladimir Ignatchenko, and Thomas Kislinger --
Cloning, expression, and purification of the glycosylated transmembrane protein, cation-dependent mannose 6-phosphate receptor, from Sf9 cells using the baculovirus system / Linda J. Olson and Nancy M. Dahms --
Bispecific antibody armed T cells to target cancer cells / Archana Thakur, Lawrence G. Lum, and Sandeep Mittal --
Immunophenotyping of live human pluripotent stem cells by flow cytometry / Daniel R. Riordon and Kenneth R. Boheler --
Detecting cell surface expression of the G protein-coupled receptor CXCR4 / Amanda M. Nevins and Adriano Marchese --
NaV channels : assaying biosynthesis, trafficking, function / Gordon F. Tomaselli and Federica Farinelli --
High-content electrophysiological analysis of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) / Chi-Wing Kong, Lin Geng, and Ronald A. Li --
Methods for evaluation of vascular endothelial cell function with transient receptor potential (TRP) channel drugs / Yung Wui Tjong and Xiaoqiang Yao --
Methods to study the signal transduction of the surface receptor tyrosine kinase TrkB in neurons / Kwok-On Lai and Nancy Y. Ip --
Polarized human retinal pigment epithelium exhibits distinct surface proteome on apical and basal plasma membranes / Vladimir Khristov, Qin Wan, Ruchi Sharma, Mostafa Lotfi, Arvydas Maminishkis, and Kapil Bharti --
Extracellular matrix molecule-based capture of mesenchymal stromal cells under flow / Teresa Massam-Wu, Stuart A. Cain, and Cay M. Kielty --
Generation of induced pluripotent stem cells from patients with COL3A1 mutations and differentiation to smooth muscle cells for ECM-surfaceome analyses / Jiaozi He, Zhihui Weng, Stanley Chun Ming Wu, and Kenneth R. Boheler --
Fabrication and mechanical properties measurements of 3D microtissues for the study of cell-matrix interactions / Prasenjit Bose, Chen Yu Huang, Jeroen Eyckmans, Christopher S. Chen, and Daniel H. Reich --
Discovery of surface target proteins linking drugs, molecular markers, gene regulation, protein networks, and disease by using a web-based platform targets-search / Bin Yan, Panwen Wang, Junwen Wang, and Kenneth R. Boheler.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1722.
Responsibility: edited by Kenneth R. Boheler, Rebekah L. Gundry.


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