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Synaptosomes : methods and protocols

Author: Kathryn M Murphy
Publisher: New York: Humana Press ; Springer, ©2018.
Series: Neuromethods, v. 141.
Edition/Format:   eBook : Document : EnglishView all editions and formats

This volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses.After examining the history that led  Read more...


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Genre/Form: Electronic books
Laboratory manuals
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Kathryn M Murphy
ISBN: 9781493987399 1493987399
OCLC Number: 1053902202
Description: 1 online resource (xi, 312 pages) : illustrations (some color).
Contents: Introduction to synaptosomes / Kathryn M. Murphy --
Victor P. Whittaker : the discovery of the synaptosome and its implications / Herbert Zimmermann --
Synaptosome preparations : which procedure should i use? / Peter R. Dunkley and Phillip J. Robinson --
Protocol for a high-throughput semiautomated preparation for filtered synaptoneurosomes / Justin L. Balsor and Kathryn M. Murphy --
Subcellular fractionation of brain tissue from small tissue explants / Véronique De-Smedt-Peyrusse, Laetitia Darriet, Pierre Trifilieff, Etienne Herzog, and Maria Florencia Angelo --
Preparation of P2 or percoll-purified synaptosomes from mammalian brain tissue / Jing Xue, Annie Quan, and Phillip J. Robinson --
Synaptosomal bioenergetics and glutamate release / David G. Nicholls --
Synaptosome bioenergetics and calcium handling : aging response / Silvia Lores-Arnaiz, Georgina Rodríguez de Lores Arnaiz, Analía G. Karadayian, and Juanita Bustamante --
Isolation of highly purified and functionally active synaptic mitochondria / Sergei V. Baranov, Nicolas K. Khattar, Svitlana Yablonska, Oxana V. Baranova, Eric S. Kretz, Timothy M. Larkin, Diane L. Carlisle, and Robert M. Friedlander --
Affinity proteomics for interactome and phosphoproteome screening in synaptosomes / Kasper Engholm-Keller, Nicolai Bache, Sushma R. Rao, Jesse R. Wark, Martin R. Larsen, Phillip J. Robinson, and Mark E. Graham --
Manipulating synapse-enriched biochemical fractions to detect a purified synaptic protein's effect on neurotransmitter release / Marianne Cilluffo, Theodore A. Sarafian, and Joseph B. Watson --
Porosomes : supramolecular structures at the synaptosome membrane involved in vesicle docking, fusion, and neurotransmitter release / Bhanu P. Jena --
SMPL synaptic membranes : nanodisc-mediated synaptic membrane mimetics expand the toolkit for drug discovery and the molecular cell biology of synapses / Riana Lo Bu, Jeffrey Clark, Thomas Dichiara, Stephen G. Sligar, and William L. Klein --
Profiling of microRNA and protein from purified synaptoneurosomes in a neurodegenerative disease model / Amrit S. Boese, Anna Majer, and Stephanie A. Booth --
Use of synaptoneurosome samples to study development and plasticity of human cortex / Caitlin R. Siu, Simon P. Beshara, Justin L. Balsor, Steven J. Mancini, and Kathryn M. Murphy --
Use of synaptosomes in studying autism spectrum disorder and other neurodevelopmental disorders / Nadeem Murtaza, Vickie Kwan, Biren Dave, and Karun K. Singh --
Flow cytometric evaluation of crude synaptosome preparation as a way to study synaptic alteration in neurodegenerative diseases / Nadia O. Postupna, Caitlin S. Latimer, C. Dirk Keene, Kathleen S. Montine, Thomas J. Montine, and Martin Darvas.
Series Title: Neuromethods, v. 141.
Responsibility: edited by Kathryn M. Murphy.


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