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Synaptosomes : methods and protocols

Author: Kathryn M Murphy
Publisher: New York: Humana Press ; Springer, ©2018.
Series: Neuromethods, v. 141.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:

This volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses.After examining the history that led  Read more...

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Genre/Form: Electronic books
Laboratory manuals
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Kathryn M Murphy
ISBN: 9781493987399 1493987399
OCLC Number: 1053902202
Description: 1 online resource (xi, 312 pages) : illustrations (some color).
Contents: Introduction to synaptosomes / Kathryn M. Murphy --
Victor P. Whittaker : the discovery of the synaptosome and its implications / Herbert Zimmermann --
Synaptosome preparations : which procedure should i use? / Peter R. Dunkley and Phillip J. Robinson --
Protocol for a high-throughput semiautomated preparation for filtered synaptoneurosomes / Justin L. Balsor and Kathryn M. Murphy --
Subcellular fractionation of brain tissue from small tissue explants / Véronique De-Smedt-Peyrusse, Laetitia Darriet, Pierre Trifilieff, Etienne Herzog, and Maria Florencia Angelo --
Preparation of P2 or percoll-purified synaptosomes from mammalian brain tissue / Jing Xue, Annie Quan, and Phillip J. Robinson --
Synaptosomal bioenergetics and glutamate release / David G. Nicholls --
Synaptosome bioenergetics and calcium handling : aging response / Silvia Lores-Arnaiz, Georgina Rodríguez de Lores Arnaiz, Analía G. Karadayian, and Juanita Bustamante --
Isolation of highly purified and functionally active synaptic mitochondria / Sergei V. Baranov, Nicolas K. Khattar, Svitlana Yablonska, Oxana V. Baranova, Eric S. Kretz, Timothy M. Larkin, Diane L. Carlisle, and Robert M. Friedlander --
Affinity proteomics for interactome and phosphoproteome screening in synaptosomes / Kasper Engholm-Keller, Nicolai Bache, Sushma R. Rao, Jesse R. Wark, Martin R. Larsen, Phillip J. Robinson, and Mark E. Graham --
Manipulating synapse-enriched biochemical fractions to detect a purified synaptic protein's effect on neurotransmitter release / Marianne Cilluffo, Theodore A. Sarafian, and Joseph B. Watson --
Porosomes : supramolecular structures at the synaptosome membrane involved in vesicle docking, fusion, and neurotransmitter release / Bhanu P. Jena --
SMPL synaptic membranes : nanodisc-mediated synaptic membrane mimetics expand the toolkit for drug discovery and the molecular cell biology of synapses / Riana Lo Bu, Jeffrey Clark, Thomas Dichiara, Stephen G. Sligar, and William L. Klein --
Profiling of microRNA and protein from purified synaptoneurosomes in a neurodegenerative disease model / Amrit S. Boese, Anna Majer, and Stephanie A. Booth --
Use of synaptoneurosome samples to study development and plasticity of human cortex / Caitlin R. Siu, Simon P. Beshara, Justin L. Balsor, Steven J. Mancini, and Kathryn M. Murphy --
Use of synaptosomes in studying autism spectrum disorder and other neurodevelopmental disorders / Nadeem Murtaza, Vickie Kwan, Biren Dave, and Karun K. Singh --
Flow cytometric evaluation of crude synaptosome preparation as a way to study synaptic alteration in neurodegenerative diseases / Nadia O. Postupna, Caitlin S. Latimer, C. Dirk Keene, Kathleen S. Montine, Thomas J. Montine, and Martin Darvas.
Series Title: Neuromethods, v. 141.
Responsibility: edited by Kathryn M. Murphy.

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