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T-cell motility : methods and protocols

Author: Navin Kumar Verma
Publisher: New York : Humana Press ; Springer, [2019].
Series: Methods in molecular biology (Clifton, N.J.), v. 1930.
Edition/Format:   eBook : Document : EnglishView all editions and formats
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Genre/Form: Electronic books
Laboratory Manual
Laboratory manuals
Additional Physical Format: Print version:
T-cell motility.
New York : Springer, c2019
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Navin Kumar Verma
ISBN: 9781493990368 1493990365
OCLC Number: 1081043964
Description: 1 online resource (x, 158 pages) : illustrations (chiefly color)
Contents: Introduction to LFA-1/ICAM-1 interactions in T-cell motility / Navin Kumar Verma and Dermot Kelleher --
Isolation of human peripheral blood T-lymphocytes / Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, and Navin Kumar Verma --
Laboratory model to study T-cell motility / Navin Kumar Verma, Shyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Atish Kizhakeyil, and Dermot Kelleher --
Quantification of T-cell migratory phenotypes using high-content analysis / Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, and Navin Kumar Verma --
Live cell imaging and analysis to capture T-cell motility in real-time / Seow Theng Ong and Navin Kumar Verma --
Three-dimensional structured illumination microscopy (3D-SIM) to dissect signaling cross-talks in motile T-cells / Seow Theng Ong, Graham D. Wright, and Navin Kumar Verma --
Real-time impedance-based detection of LFA-1-stimulated T-cell transwell chemotaxis / Praseetha Prasannan and Navin Kumar Verma --
Quantitative real-time PCR for Evaluating Transcriptional Changes in T-Lymphocytes / Atish Kizhakeyil and Navin Kumar Verma --
GapmeR-mediated gene silencing in motile T-cells / Mobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Somaraju Chalasani, Atish Kizhakeyil, and Navin Kumar Verma --
Live imaging of resident T-cell migration in human lymphoid tissue slices using confocal microscopy / Emmanuel Donnadieu, Yvonne Michel, and Martin-Leo Hansmann --
Phosphoprotein enrichment for protein analysis in motile T-lymphocytes / Praseetha Prasannan, Rathi Saravanan, and Navin Kumar Verma --
Immunometabolomic phenotyping of motile T-cells / Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, and Navin Kumar Verma --
Profiling activity of cellular kinases in migrating T-cells / Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy, Navin Kumar Verma, and WimVanden Berghe --
Biotinylation assay to determine LFA-1 recycling in motile T-lymphocytes / Malin Samuelsson and Lena M. Svensson --
Protocol to study T-cell signaling in an immune synapse by microscopy / Mobashar Hussain Urf Turabe Fazil, Pankaj Kumar, and Navin Kumar Verma --
Enzyme-linked immunosorbent assay for T-cell dependent immunogenicity assessment of therapeutic peptides / Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, and Navin Kumar Verma --
Modified intravital microscopy to assess vascular health and T-cell motility / Geoffrey W. Payne, Kevin Mitchell, and Stephanie L. Sellers --
Computational analysis of protein-protein interactions in motile T-cells / Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khurshid Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, and Navin Kumar Verma.
Series Title: Methods in molecular biology (Clifton, N.J.), v. 1930.
Responsibility: edited by Navin Kumar Verma.

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