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A time-shared dual optical trap with sub-nanometer image-based position resolution for hybrid instruments

Author: Jessica Killian; Michelle D Wang
Publisher: Ann Arbor, Michigan : ProQuest Information and Learning, 2017. ©2017
Dissertation: Ph. D. Cornell University January 2017
Edition/Format:   Thesis/dissertation : Document : Thesis/dissertation : eBook   Computer File : English
Summary:
Optical trapping is a powerful and sensitive technique that enables the direct measurement and manipulation of biological samples on the piconewton and nanometer scales. In examining the evolution and maturation of optical trapping instrumentation in the field of single-molecule biophysics, two main thrusts become apparent; a subset of instruments have aimed to push the limits of resolution, seeking to capture the  Read more...
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Details

Genre/Form: Academic theses
Material Type: Document, Thesis/dissertation, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: Jessica Killian; Michelle D Wang
OCLC Number: 1003395764
Description: 1 online resource (195 leaves) : illustrations
Responsibility: by Jessica Killian.

Abstract:

Optical trapping is a powerful and sensitive technique that enables the direct measurement and manipulation of biological samples on the piconewton and nanometer scales. In examining the evolution and maturation of optical trapping instrumentation in the field of single-molecule biophysics, two main thrusts become apparent; a subset of instruments have aimed to push the limits of resolution, seeking to capture the finest details of biomechanical processes, while a second subset of instruments have explored the integration of optical trapping with other measurement techniques, with the goal of creating multidimensional, hybrid instruments. Unfortunately, many hybrid trapping instruments are forced to sacrifice measurement resolution, or relinquish their measurement capabilities entirely, when the combination of techniques cannot accommodate the stringent demands of traditional optical trapping detection methods. Thus, the full potential of hybrid optical trapping instruments has remained elusive. We have taken on this challenge by developing a highly-versatile optical tweezers instrument that can achieve sub-nanometer position resolution without the use of a second microscope objective, making high-resolution optical trapping measurements uniquely compatible with a broad array of bulky and/or opaque sample-side devices. Our instrument consists of a time-shared, dual optical trap implemented in an entirely custom-built, free-standing fluorescence microscope, with camera-based position detection. We have developed a robust and accurate set of tracking techniques that allow us to make the first direct measurements of relative bead displacement within an optical trap with sub-nanometer resolution using image tracking. These capabilities pave the way for the next generation of optical tweezer hybrids, which we envision as versatile tools that can integrate multiple measurement modalities and complex substrates without sacrificing resolution, to expand the breadth and clarity of our window into the microscopic and sub-microscopic biological worlds. ...

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