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Ubiquitin and ubiquitin-like protein modifiers

Author: Mark W Hochstrasser
Publisher: Cambridge, MA : Academic Press, an imprint of Elsevier, 2019.
Series: Methods in enzymology, v. 618.
Edition/Format:   Print book : English : First editionView all editions and formats
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Document Type: Book
All Authors / Contributors: Mark W Hochstrasser
ISBN: 9780128163597 0128163593
OCLC Number: 1101483073
Description: xix, 410 pages : illustrations (some color) ; 24 cm
Contents: Semisynthesis of ubiquitinated histone H2B with a native or nonhydrolyzable linkage / Michael Morgan, Muhammad Jbara, Ashraf Brik and Cynthia Wolberger --
Dual-color pulse-chase ubiquitination assays to simultaneously monitor substrate priming and extension / Daniel C. Scott and Brenda A. Schulman --
A genetic approach to study polyubiquitination in Saccharomyces cerevisiae / Fernando Meza-Gutierrez, Deniz Simsek and David P. Toczyski --
Enzymatic preparation of monoubiquitinated FANCD2 and FANCI proteins / Viduth K. Chaugule, Connor Arkinson, Rachel Toth and Helen Walden --
Methods to measure ubiquitin chain length and linkage / Fumiaki Ohtake, Hikaru Tsuchiya, Keiji Tanaka and Yasushi Saeki --
Detection of ubiquitination activity and identification of ubiquitinated substrates using TR-TUBE / Yukiko Yoshida, Yasushi Saeki, Hikaru Tsuchiya and Keiji Tanaka --
Methods to study phosphoribosylated ubiquitin ligation and removal / Jiazhang Qiu and Zhao-Qing Luo --
Biochemical characterization of SUMO-conjugating enzymes by in vitro sumoylation assays / Nathalie Eisenhardt, Dragana Ilic, Easa Nagamalleswari and Andrea Pichler --
Methods to study SUMO dynamics in yeast / Stefan Pabst, Lennard-Maximilian Döring, Natasha Petreska and R. Jürgen Dohmen --
Approaches for investigating the extracellular signaling function of ISG15 / Caleb D. Swaim, Larissa A. Canadeo and Jon M. Huibregtse --
Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10 / Annette Aichem, Annika N. Boehm, Nicola Catone, Gunter Schmidtke and Marcus Groettrup --
Methods to analyze STUbL activity / Emma Branigan, Anna Plechanovová and Ronald T. Hay --
Quantitative analysis of USP activity in vitro / Shreya Dharadhar, Robbert Q. Kim, Michael Uckelmann and Titia K. Sixma --
Evaluating enzyme activities and structures of DUBs / Jonathan N. Pruneda and David Komander --
Purification and functional characterization of the DUB domain of SdeA / Kedar Puvar, Shalini Iyer, Michael J. Sheedlo and Chittaranjan Das --
Profiling DUBs and Ubl-specific proteases with activity-based probes / Paul P. Geurink, Gerbrand J. van der Heden van Noort, Monique P.C. Mulder, Robert C.M. Knaap, Marjolein Kikkert and Huib Ovaa --
Assays of SUMO protease/isopeptidase activity and function in mammalian cells and tissues / Kathrin Kunz, Stefan Müller and Luca Mendler.
Series Title: Methods in enzymology, v. 618.
Responsibility: edited by Mark Hochstrasser.

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