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Understanding light microscopy

Author: J B Sanderson
Publisher: Hoboken, NJ : John Wiley & Sons, 2019.
Series: RMS-Wiley series.
Edition/Format:   eBook : Document : EnglishView all editions and formats
Summary:
Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This  Read more...
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Genre/Form: Electronic books
Additional Physical Format: Print version:
Sanderson, J.B. (Jeremy B.).
Understanding light microscopy.
Hoboken, NJ : John Wiley & Sons, 2018
(DLC) 2018004188
Material Type: Document, Internet resource
Document Type: Internet Resource, Computer File
All Authors / Contributors: J B Sanderson
ISBN: 9781118696743 1118696743 9781118696736 1118696735 9781118696811 1118696816
OCLC Number: 1027728898
Description: 1 online resource (xxix, 815 pages).
Contents: Our eyes and the microscope --
Light --
Basic microscope optics --
Microscope design --
Ergonomics --
Optical aberrations of the microscope --
The microscope objective --
Condensers & eyepieces --
Illumination in the microscope --
Diffraction and image formation in microscopy --
Contrast generation & enhancement --
Reflected-light microscopy --
Polarised-light microscopy : part 1, theory --
Polarised-light microscopy : part 2, applied --
Fluorescence microscopy --
Fluorophores & fluorescent proteins --
Optical sectioning & confocal microscopy --
Operating the confocal microscope --
Light-sheet microscopy --
Bleed-through & spectral unmixing --
Deconvolution --
Multi-photon microscopy --
Total internal reflection fluorescence microscopy --
Frap and fret --
Colocalisation --
Super-resolution fluorescence microscopy --
Choosing a microscope platform and core imaging facilities --
Biological specimen preparation --
Materials specimen preparation --
Recording the image : part 1, theory --
Recording the image : part 2, applied.
Series Title: RMS-Wiley series.
Responsibility: Jeremy Sanderson.

Abstract:

This textbook introduces readers to the modern light microscope. It can either be used alone or in conjunction with a practical course. The book begins with an account of how the light microscope  Read more...

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