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Genre/Form: | Electronic books |
---|---|
Additional Physical Format: | Printed edition: |
Material Type: | Document, Internet resource |
Document Type: | Internet Resource, Computer File |
All Authors / Contributors: |
Loredano Pollegioni; Stefano Servi |
ISBN: | 9781617793318 1617793310 9781617793301 1617793302 |
OCLC Number: | 756512314 |
Language Note: | English. |
Description: | 1 online resource (xiv, 409 pages) : illustrations. |
Contents: | Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases -- Multistep enzyme catalyzed reactions for unnatural amino acids -- Enzymatic production of enantiopure amino acids from mono-substituted hydantoin substrates -- Preparation of glutamate analogues by enzymatic transamination -- Carbon-carbon bond-forming enzymes for the synthesis of non-natural amino acids -- Engineering cyclic amidases for non-natural amino acid synthesis -- NMR analysis of unnatural amino acids in natural antibiotics -- Site-specific incorporation of unnatural amino acids as probes for protein conformational changes -- Application of unnatural amino acids to the de novo design of selective antibiotic peptides -- Use of unnatural amino acids to probe structure-activity relationships and mode-of-action of antimicrobial peptides -- Experimental methods for scanning unnatural amino acid mutagenesis -- Genetic incorporation of unnatural amino acids into proteins in yeast -- Site-specific incorporation of unnatural amino acids into proteins in mammalian cells -- Incorporation of unnatural non-alpha-amino acids into the N-terminus of proteins in a cell-free translation system -- Site-specific modification of proteins by the staudinger-phosphite reaction -- Hplc methods for determination of D-aspartate and N-methyl-D-aspartate -- Estimation of chronological age from the racemization rate of L- and D-aspartic acid: How to completely separate enantiomers from dentin -- Enzymatic detection of D-amino acids -- An enzymatic-HPLC assay to monitor endogenous D-serine release from neuronal cultures -- Electrophysiological analysis of the modulation of NMDA-receptors function by D-serine and glycine in the central nervous system -- Biosensors for d-amino acid detection -- Analysis of D-[beta]-aspartyl isomers at specific sites in proteins -- Nutritional value of D-amino acids, d-peptides, and amino acid derivatives in mice -- Preparation and assay of recombinant serine racemase -- Assay of amino acid racemases -- Assays of D-amino acid oxidases -- Enzymes acting on D-amino acid containing peptides. |
Series Title: | Methods in molecular biology (Clifton, N.J.), v. 794. |
Responsibility: | edited by Loredano Pollegioni, Stefano Servi. |
Abstract:
Divided into convenient sections, this book contributes to directing the reader's attention to the many fields of growing scientific interest in non-natural amino acids. It includes methods and protocols, and provides step-by-step readily reproducible results.
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Related Subjects:(32)
- Amino acids.
- Human Anatomy & Physiology.
- Health & Biological Sciences.
- Animal Biochemistry.
- Molecular Structure.
- Protein Engineering.
- Mutagenesis.
- Amino Acids, Peptides, and Proteins.
- Publication Formats.
- Molecular Sequence Data.
- Chemicals and Drugs.
- Genetic Engineering.
- Biochemical Phenomena.
- Documentation.
- Publication Characteristics.
- Genetic Phenomena.
- Genetic Techniques.
- Chemical Phenomena.
- Information Services.
- Information Science.
- Phenomena and Processes.
- Investigative Techniques.
- Analytical, Diagnostic and Therapeutic Techniques and Equipment.
- Amino Acid Sequence.
- Amino Acid Substitution.
- Laboratory Manuals.
- Amino Acids.
- Life sciences
- Biochemistry
- Enzymes
- Biochemistry, general
- Enzymology