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Uporaba sinhrotronske svetlobe pri študiju strukture proteinov = Synchrotron radiation in macromolecular crystallography
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Uporaba sinhrotronske svetlobe pri študiju strukture proteinov = Synchrotron radiation in macromolecular crystallography

Author: Saša Jenko
Edition/Format:   Article : Slovenian
Publication:Vakuumist, Letn. 22, št. 1 (2002), str. 10-15
Summary:
Synchrotron radiation has become a major structural research tool across the world in numerous scientific areas. It is a critical component for many of these developments, and it is essential for high-throughput crystallography. The source characteristics of modern synchrotrons are ideally matched to a number of difficult problems im macromolecular crystallography. Recent studies on viruses, membrane proteins and
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Document Type: Article
All Authors / Contributors: Saša Jenko
ISSN:0351-9716
OCLC Number: 441833022
Description: str. 10-15.
Other Titles: Synchrotron radiation in macromolecular crystallography
Responsibility: Saša Jenko.

Abstract:

Synchrotron radiation has become a major structural research tool across the world in numerous scientific areas. It is a critical component for many of these developments, and it is essential for high-throughput crystallography. The source characteristics of modern synchrotrons are ideally matched to a number of difficult problems im macromolecular crystallography. Recent studies on viruses, membrane proteins and ribosomes give clear examples of the power of modern macromolecular crystallography using synchrotrone radiation. The detailed structural information obtained enables the relationship between structure and function to be addressed with confidence.

Sinhrotronsko sevanje se zadnjih dvajset let uporablja za raziskave v številnih tehniških in naravoslovnih vedah, kot so fizika, kemija, fizikalna metalurgija, biokemija, farmacija, medicina in druge. Sinhrotronsko sevanje je postalo eno izmed glavnih orodij v biokemiji za strukturne študije proteinov; je nujno potrebno za razvoj "high-throughput" kristalografije, ki bo omogočila avtomatizirano reševanje novih struktur množice proteinov, ko so rezultat hitrega razvoja področja genomike (določevanja zaporedja genov dednega zapisa) in proteomike (določevanja struktur proteinov, ki jih ti geni zapisujejo). Poleg tega moderni sinhrotroni omogočajo reševanje težjih problemov v makromolekulski kristalografiji, kot so strukture virusov, membranskih proteinov idr. Podatki, pridobljeni z uporabo sinhrotronskega sevanja, omogočajo natančnejšo določitev strukture proteinov in s tem posredno njihove funkcije.

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